论文推荐-15-外泌体 · Jun 11 15:16

{"success": true, "name": "paper-recommender", "description": "每日定时推送高质量论文推荐 — PubMed检索 → Hermes+Claude双重评估 → 仅推最优1篇 → HTML深度解读", "tags": ["PubMed", "论文推荐", "Claude评估", "文献解读", "科研"], "related_skills": [], "content": "---\nname: paper-recommender\ndescription: \"每日定时推送高质量论文推荐 — PubMed检索 → Hermes+Claude双重评估 → 仅推最优1篇 → HTML深度解读\"\nversion: 2.1.0\nauthor: Hermes\ntags: [PubMed, 论文推荐, Claude评估, 文献解读, 科研]\n---\n\n# 📚 每日论文推荐工作流 (Paper Recommender)\n\n## 职责\n根据用户的6个研究兴趣方向，每日定时从PubMed检索近期高质量论文，经过Hermes+Claude双重评估后，**只选最优的1篇**，下载PDF+提取原图，制作公众号风格的HTML深度解读文件并推送。\n\n## 用户研究方向\n1. 帕金森病(PD) / GZR18 / α-synuclein\n2. 神经炎症 / 小胶质细胞 / 星形胶质细胞\n3. 细胞死亡方式 (Mitoxyperiosis / 铁死亡 / 焦亡 / 泛素化降解)\n4. 外泌体 / 细胞外囊泡 / 神经退行性疾病\n5. 脑缺血 / 脑卒中 / 神经保护\n6. 生信分析 / 单细胞测序 / 多组学\n\n## 定时推送安排\n\n| 时间 | 主题 | 关键词 |\n|:----:|------|--------|\n| 08:00 | PD/帕金森病 | Parkinson's disease, α-synuclein, MPTP, dopamine neuron |\n| 11:00 | 神经炎症/胶质细胞 | neuroinflammation, microglia, astrocyte, NLRP3 |\n| 13:00 | 细胞死亡/信号通路 | cell death, mitoxyperiosis, ferroptosis, ubiquitination |\n| 15:00 | 外泌体/细胞通讯 | exosome, extracellular vesicle, neural, brain |\n| 17:00 | 脑缺血/神经保护 | cerebral ischemia, stroke, neuroprotection, MCAO |\n| 19:00 | 生信/前沿技术 | single-cell, multi-omics, bioinformatics, spatial transcriptomics |\n\n## ⚠️ 已知工具陷阱\n\n### 文件系统隔离（重要！）\n`execute_code` 运行在沙箱环境中，**不能直接访问** `terminal` 工具写入 `/tmp/` 或 `$HOME/` 的文件。反之亦然。\n- ❌ 错误做法：terminal写入`/tmp/fig1_b64.txt`，然后用execute_code的`open()`读取 → 文件不存在\n- ✅ 正确做法1：所有数据处理在terminal中完成（Python脚本通过`python script.py`执行）\n- ✅ 正确做法2：将数据处理写成`.py`文件写入磁盘，再用terminal(`python path/to/script.py`)执行\n\n### 大文件HTML生成\n当HTML嵌入base64图片时（5张Nature原图 ≈ 4MB），`write_file`会因大小限制失败。\n- ❌ 不要直接用write_file写4MB+的HTML\n- ✅ 策略：写一个Python builder脚本到磁盘（`write_file → build_html.py`），然后`terminal(\"python build_html.py\")`执行它\n\n## 执行流程（完整版）\n\n### Step 1: PubMed检索 — 两阶段策略\n\n**方式：Python urllib**（推荐，避免curl的SSL/编码问题）\n```python\nimport urllib.request, urllib.parse, json\nparams = {\n 'db': 'pubmed', 'term': query,\n 'mindate': '<3个月前>', 'maxdate': '<今天>',\n 'datetype': 'pdat', 'retmax': 15, 'retmode': 'json'\n}\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?' + urllib.parse.urlencode(params)\n```\n\n**阶段A — 主题搜索**：用关键词搜该方向近期论文\n**阶段B — 高IF期刊过滤**：用主题词 + `(\"Nature\"[Journal] OR \"Cell\"[Journal] OR ...)` 格式搜\n\n⚠️ 关键陷阱：\n- `sort=date` 被PubMed忽略（返回warning），不要用\n- 期刊名必须用 `\"全称\"[Journal]` 格式，裸词会被翻译为MeSH词条\n- 复杂查询在curl中容易URL编码失败，优先用Python\n\n### Step 2: 获取论文详情并初筛\n\n```python\n# 获取元数据\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&id=<PMIDs>&retmode=json'\n# 获取摘要\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=<PMIDs>&retmode=xml'\n```\n\n**初筛硬性门槛**（必须同时满足）：\n1. IF > 10 或领域顶刊\n2. 2026年3月后发表\n3. 与用户研究方向直接相关\n4. 原创研究（pubtype不含Review）\n5. 非 News & Views / Letter / 编辑评论\n\n### Step 3: Claude Code深度评估（Hermes + Claude 双重评判）\n\n将经过初筛的候选论文交给 Claude Code 严格评估：\n```\nclaude -p \"严格评估以下论文，从5个维度各评1-10分...\"\n```\n\n**5维度评分**：\n1. **逻辑完整性** (1-10)：完整正反验证（gain+loss of function）、KO/KI模型\n2. **方法学水平** (1-10)：前沿技术（单细胞测序、冷冻电镜、活细胞成像、TurboID等）\n3. **创新性** (1-10)：新概念/新机制/新方法，首次报道\n4. **可借鉴性** (1-10)：实验设计是否值得用户在自己的课题中参考 **← 这是最关键的维度**\n5. **临床转化潜力** (1-10)：体内验证或治疗意义\n\n### Step 4: 唯一推荐（核心规则）\n\n- Claude评分 < 30分（平均<6分）或任何单维度 < 5分 → **不推荐**，跳过\n- 多个候选都达标 → **只选最优的1篇**，不要让用户选\n- Hermes再次确认质量 + 相关性 + 可借鉴性 → 只有双方都确认才通过\n- **如果这篇研究方向不匹配（如planarian flatworm研究），即使评分高也不能推**\n\n### Step 5: 下载PDF + 提取Figure图片\n\n**PDF下载**：\n- Nature: `https://www.nature.com/articles/{article_id}.pdf`\n- Cell: (可能需要其他方式)\n- 注意：部分期刊有付费墙，PDF可能无法下载，此时跳过\n\n**Figure提取（Nature模式 — 已验证 2026-06）**：\n```bash\n# 1. 从HTML中提取所有figure图片URL\ncurl -s -L -H \"User-Agent: Mozilla/5.0\" \"https://www.nature.com/articles/{article_id}\" \\\n | grep -oP 'https://media\\.springernature\\.com/[^\"'\"'\"'<>]+s41586[^\"'\"'\"'<>]*\\.(?:png|jpg|webp)' \\\n | sort -u\n\n# 2. 下载每个figure为base64（用于HTML嵌入）\nFIG_URL=\"https://media.springernature.com/full/springer-static/image/art%3A10.1038%2F{article_id}/MediaObjects/41586_{year}_{article_num}_Fig{N}_HTML.png\"\ncurl -s \"$FIG_URL\" -H \"User-Agent: Mozilla/5.0\" | base64 -w 0\n```\n\n**分辨率选择**：\n- `/full/` — 最高分辨率（推荐用于Fig1）\n- `/lw1200/` — 宽1200px（其余Figures用此即可）\n- `/m685/` — 宽685px（小尺寸）\n\n**已验证的图片URL模式**（article_id = s41586-026-10597-2的实例）：\n```\nURL前缀: https://media.springernature.com/{size}/springer-static/image/art%3A10.1038%2F{article_id}/MediaObjects/\n文件: 41586_2026_10597_Fig{N}_HTML.png\n```\n\n- 参考 `references/figure-download-patterns.md` 获取各期刊的详细URL模式\n\n### Step 6: 制作公众号风格HTML深度解读 — Python Builder模式\n\nHTML文件要求：\n1. 所有图片用JS变量(base64)嵌入，不使用file://协议\n2. 仿公众号排版（简洁卡片风格）\n3. 每条结果配\"复现建议\"（🔬 复现建议：...）\n4. 包含论文原图 + 逐图解读 + 文章小结\n5. 保存到桌面，文件命名规范：`{主题关键词}_文献解读.html`\n\n**实现方法**（大文件HTML必须用此方式）：\n由于HTML含5张base64图片（~4MB），不能直接用`write_file`写入。\n```python\n# 1. 先读取base64数据（terminal工具写入的文件）\nfig_b64 = {}\nfor i in range(1, 6):\n with open(f\"/c/Users/<user>/fig{i}_b64.txt\", \"r\") as f:\n fig_b64[f\"fig{i}\"] = f.read().strip()\n\n# 2. 构建HTML字符串（用f-string嵌入base64）\nhtml = f'''...<img src=\"data:image/png;base64,{fig_b64['fig1']}\">...'''\n\n# 3. 写入文件\nwith open(\"C:/Users/<user>/Desktop/细胞死亡_文献解读.html\", \"w\", encoding=\"utf-8\") as f:\n f.write(html)\n```\n\n**流程总结**（6步）：\n1. terminal中`curl | base64 -w 0 > /tmp/fig{N}_b64.txt` 下载所有图片\n2. 用`write_file`将HTML builder脚本写入磁盘（不含base64数据，仅模板）\n3. terminal中执行该Python脚本（读取base64文件 + 构建完整HTML + 写入桌面）\n4. 验证HTML和PDF文件存在：`ls -la /c/Users/<user>/Desktop/`\n\n**HTML模板风格指南**：\n- 头部：渐变深蓝背景 + 白色文字 + 标签徽章\n- 评分卡片：flex网格布局，5维度评分条\n- 图文解读：Fig依次排列，每个配canvas+caption+复现建议\n- 文章小结：渐变紫色卡片\n- 参考 `references/html-template-structure.md` 获取模板框架\n\n### Step 7: 微信推送\n\n**格式要求**：\n```\n🔥 {时间} {主题} 论文推荐\n\n🏆 标题...\n📅 日期 | 📰 期刊\n⭐ Claude评分：X/50\n💡 推荐理由：...\n\n（解读HTML已生成，请查收）\n```\n\n**如果当天没有好论文**：\n```\n📭 {时间} {主题}\n今天这个方向没有找到特别适合你的论文，下次继续关注。\n```\n\n## 核心原则\n- **只推1篇** —— 不要让用户从多个候选中选择\n- **宁缺毋滥** —— 没有好论文就不推，不强塞\n- **Hermes + Claude 双重把关** —— 都确认完美适合才推\n- **不推荐综述**（除非极高价值）\n- **不推荐与用户研究方向无关的论文**（即使评分高）\n- **推送语言用中文**，但保留英文标题和期刊名\n\n## 参考文件\n- `references/pubmed-API-patterns.md` — PubMed E-utilities API详细笔记\n- `references/figure-download-patterns.md` — Nature/Cell/Elsevier等期刊的图片URL提取模式\n", "path": "productivity\\paper-recommender\\SKILL.md", "skill_dir": "C:\\Users\\forgase\\AppData\\Local\\hermes\\skills\\productivity\\paper-recommender", "linked_files": {"references": ["references\\figure-download-patterns.md", "references\\html-template-structure.md", "references\\pubmed-API-patterns.md"]}, "usage_hint": "To view linked files, call skill_view(name, file_path) where file_path is e.g. 'references/api.md' or 'assets/config.yaml'", "required_environment_variables": [], "required_commands": [], "missing_required_environment_variables": [], "missing_credential_files": [], "missing_required_commands": [], "setup_needed": false, "setup_skipped": false, "readiness_status": "available"}

{"success": true, "name": "paper-recommender", "file": "references/figure-download-patterns.md", "content": "# 学术期刊Figure下载模式速查\n\n## Nature 系列\n**已验证模式（2026年6月，article_id = s41586-026-10597-2）**\n\n### URL结构\n```\n文章页: https://www.nature.com/articles/{article_id}\nFigure URL: https://media.springernature.com/{size}/springer-static/image/\n art%3A10.1038%2F{article_id}/MediaObjects/\n {journal_id}_{year}_{article_num}_Fig{N}_HTML.png\n```\n\n### 关键参数\n- `{article_id}`: DOI中`/`后面的部分，如 `s41586-026-10597-2`\n- `{journal_id}`: 期刊对应数字ID，如 Nature=41586\n- `{year}`: 4位年份\n- `{article_num}`: 文章编号\n- `{N}`: Figure编号（1-7）\n\n### 实例（已测试通过）\n```\narticle_id = s41586-026-10597-2\njournal_id = 41586\nyear = 2026\narticle_num = 10597\n\nFig1 全尺寸:\nhttps://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41586-026-10597-2/MediaObjects/41586_2026_10597_Fig1_HTML.png\n\nFig2 中等尺寸:\nhttps://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41586-026-10597-2/MediaObjects/41586_2026_10597_Fig2_HTML.png\n```\n\n### 快速提取命令（curl + grep）\n```bash\n# 1. 从HTML提取所有Figure URL\ncurl -s -L -H \"User-Agent: Mozilla/5.0\" \\\n \"https://www.nature.com/articles/{article_id}\" \\\n | grep -oP 'https://media\\.springernature\\.com/[^\"'\"'\"'<>]+s41586[^\"'\"'\"'<>]*\\.(?:png|jpg|webp)' \\\n | sort -u\n\n# 2. 下载某张Figure为base64（用于HTML嵌入）\nFIG_URL=\"https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2F{article_id}/MediaObjects/41586_{year}_{article_num}_Fig{N}_HTML.png\"\ncurl -s \"$FIG_URL\" -H \"User-Agent: Mozilla/5.0\" | base64 -w 0 > /tmp/fig{N}_b64.txt\n```\n\n### 分辨率控制\n| 前缀 | 尺寸 | 说明 |\n|------|------|------|\n| `/full/` | 全尺寸 | 最高分辨率，推荐用于Fig1 |\n| `/lw1200/` | 宽1200px | 中等尺寸，适用于其余Figures |\n| `/m685/` | 宽685px | 小尺寸 |\n| `/w215h120/` | 215x120px | 缩略图 |\n\n### 已测试的期刊ID\n| 期刊 | ID |\n|------|----|\n| Nature | 41586 |\n| Nature Communications | 41467 |\n| Cell Death & Differentiation | 41418 |\n| Scientific Reports | 41598 |\n| Cell Discovery | 41420 |\n\n## Cell Press / Elsevier 系列\n**注意：Cell Press有强付费墙，以下模式仅供参考**\n\n### 典型URL模式\n```\nhttps://www.cell.com/cms/10.1016/j.{journal_abbr}.{year}.{article_id}/asset/\n{hash}/main.assets/gr{N}_large.jpg\n```\n\n### 备选CDN路径\n```\nhttps://els-jbs-prod-cdn.jbs.elsevierhealth.com/journals/{journal_abbr}/\n...\n```\n- Cell Press 2025年起对脚本访问限制严格（403）\n- 建议通过 PubMed Central OA 获取，或使用付费订阅机构的访问\n\n## PMC / PubMed Central 开放获取\n```\nhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC{pmcid}/bin/{filename}\n```\n- 仅对开放获取文章有效\n\n## 通用最佳实践\n\n1. **先获取文章HTML页面**，从中提取Figure图片链接\n2. **优先使用全尺寸版本**（替换路径中的尺寸限定词）\n3. **base64嵌入**到HTML中，避免file://协议被浏览器拦截\n4. **文件命名**：`Figure{N}_{简短描述}.png`\n5. **注意付费墙**：3xx/4xx响应 → 放弃，不要反复重试\n6. **Nature PDF下载**：`https://www.nature.com/articles/{article_id}.pdf` — 已验证可下载（2026-06）\n", "file_type": ".md"}

{"success": true, "name": "paper-recommender", "file": "references/html-template-structure.md", "content": "# 公众号风格HTML解读 — 模板结构\n\n## 文件架构\n\n```\n<!DOCTYPE html>\n├── <head>\n│ ├── 字符集/视口\n│ └── 样式（全部内联<style>）\n│ ├── 全局重置\n│ ├── .header — 渐变深蓝背景\n│ ├── .card — 白色圆角卡片\n│ ├── .section-title — 左边框强调标题\n│ ├── .score-bar / .score-item — 评分网格\n│ ├── .figure-box — 图片容器\n│ ├── .reproduce-tip — 蓝色复现建议\n│ ├── .highlight — 黄色高亮框\n│ └── .summary-box — 紫色渐变结论卡\n├── <body>\n│ ├── .header\n│ │ ├── 标签徽章（🔥 今日推荐 + 主题标签）\n│ │ ├── h1 中文标题\n│ │ ├── p 英文标题\n│ │ └── p 📅日期 | 📰期刊 | IF\n│ ├── .container\n│ │ ├── .card (评分) — 5维度×flex网格\n│ │ ├── .card (速览) — 摘要+高亮框\n│ │ ├── .card (团队) — 作者信息\n│ │ ├── .card (图文) — Figure×5 含canvas+caption+🔬复现建议\n│ │ ├── .card (评述) — ✅优势/⚠️缺憾/🔬借鉴\n│ │ ├── .summary-box (小结)\n│ │ └── .card (元数据) — DOI/关键词/时间\n│ └── <script>\n│ ├── const fig1_b64 = \"...\" (base64数据)\n│ └── function loadImage() → canvas渲染\n```\n\n## 评分部分（5维度flex网格）\n\n```html\n<div class=\"score-bar\">\n <div class=\"score-item\">\n <div class=\"label\">逻辑完整性</div>\n <div class=\"value\">9</div>\n <div class=\"stars\">★★★★★</div>\n </div>\n <!-- 重复 ×5 -->\n</div>\n<!-- 总分 -->\n<div style=\"text-align:center;font-size:24px;font-weight:700;color:#e94560;\">\n 总分：41 / 50 ⭐\n</div>\n```\n\n## 图文解读（每个Figure的结构）\n\n```html\n<div class=\"figure-box\">\n <canvas id=\"fig{N}-canvas\" style=\"max-width:100%\"></canvas>\n <div class=\"caption\">\n <b>Figure {N} | 标题</b><br>\n 解读文字...\n <div style=\"margin-top:8px;\">\n <b>🔬 复现建议：</b>具体建议...\n </div>\n </div>\n</div>\n<div class=\"divider\"></div>\n```\n\n## 图片嵌入方案\n\n```javascript\n// 1. base64数据存为JS变量（在<script>开头）\nconst fig1_b64 = \"iVBORw0KGgo...\";\n\n// 2. canvas渲染（onload时自适应尺寸）\nfunction loadImage(canvasId, b64) {\n var canvas = document.getElementById(canvasId);\n var ctx = canvas.getContext(\"2d\");\n var img = new Image();\n img.onload = function() {\n canvas.width = img.width;\n canvas.height = img.height;\n ctx.drawImage(img, 0, 0);\n };\n img.src = \"data:image/png;base64,\" + b64;\n}\n```\n\n## 关键样式常量\n\n| 元素 | 颜色 |\n|------|------|\n| 头部渐变 | `#1a1a2e → #0f3460` |\n| 标签徽章 | `#e94560` |\n| 卡片阴影 | `0 2px 12px rgba(0,0,0,0.08)` |\n| 评分数字 | `#e94560` |\n| 复选框背景 | `#fff8e1` + 左边框 `#ffc107` |\n| 复现建议 | `#e3f2fd` |\n| 小结卡片 | `#667eea → #764ba2` |\n| 标题左边框 | `#e94560`, 4px |\n\n## 文件命名规则\n\n```\n{主题关键词}_文献解读.html\n```\n示例：`细胞死亡_文献解读.html`、`帕金森_文献解读.html`\n\n## 注意事项\n\n1. canvas渲染：需要`onload`回调，不能直接在`<img>`上设置src（尺寸自适应问题）\n2. 所有图片必须是base64嵌入，不能用file://协议（浏览器安全策略阻止）\n3. 卡片间用`.card`统一间距（margin-bottom:20px）\n4. 长文本用`text-indent:2em`首行缩进增强可读性\n", "file_type": ".md"}

{"success": true, "name": "paper-recommender", "file": "references/pubmed-API-patterns.md", "content": "# PubMed E-utilities API 实战笔记\n\n## 搜索API (esearch)\n\n### 基础结构\n```\nbase: https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi\nparams:\n db=pubmed\n term=<查询表达式>\n mindate/maxdate — 日期范围\n datetype=pdat (publication date)\n retmax=N — 最大返回数\n retmode=json\n```\n\n### 已验证的关键查询模式\n\n**1. 主题搜索 + 高影响因子期刊过滤**\n```python\nquery = 'Parkinson AND (\"Nat Neurosci\"[Journal] OR \"Neuron\"[Journal] OR \"Nat Med\"[Journal])'\n```\n- 期刊名必须用 `\"全称\"[Journal]` 格式\n- 不加 `[Journal]` 标签时，`Neuron` 被翻译为 MeSH 的 neurons（神经元）\n- `Brain` 被翻译为 MeSH 的 brain（大脑解剖结构）\n\n**2. 细胞死亡方向搜索（已验证）**\n```python\nquery = '(ferroptosis[Title/Abstract] OR pyroptosis[Title/Abstract] OR ' + \\\n 'necroptosis[Title/Abstract] OR PANoptosis[Title/Abstract] OR ' + \\\n 'cuproptosis[Title/Abstract] OR mitophagy[Title/Abstract]) ' + \\\n 'AND (Nature[Journal] OR \"Cell\"[Journal] OR \"Nat Cell Biol\"[Journal] OR ' + \\\n '\"Cell Death Differ\"[Journal] OR \"Nat Commun\"[Journal])'\n```\n这个模式成功返回了 Nature 的精胺/铁死亡论文。\n\n**3. 已测试的有效期刊标签**\n| 期刊 | 标签 |\n|------|------|\n| Nature | `\"Nature\"[Journal]` |\n| Nature Communications | `\"Nat Commun\"[Journal]` |\n| Nature Medicine | `\"Nat Med\"[Journal]` |\n| Neuron | `\"Neuron\"[Journal]` |\n| Nature Neuroscience | `\"Nat Neurosci\"[Journal]` |\n| Science Advances | `\"Sci Adv\"[Journal]` |\n| Cell | `\"Cell\"[Journal]` |\n| Cell Reports | `\"Cell Rep\"[Journal]` |\n| Cell Death & Differentiation | `\"Cell Death Differ\"[Journal]` |\n| EMBO Journal | `\"EMBO J\"[Journal]` |\n| Molecular Neurodegeneration | `\"Mol Neurodegener\"[Journal]` |\n| EBioMedicine | `\"EBioMedicine\"[Journal]` |\n| Acta Neuropathologica | `\"Acta Neuropathol\"[Journal]` |\n| Brain | `\"Brain\"[Journal]` |\n| Lancet Neurology | `\"Lancet Neurol\"[Journal]` |\n| Developmental Cell | `\"Dev Cell\"[Journal]` |\n| Nature Structural & Molecular Biology | `\"Nat Struct Mol Biol\"[Journal]` |\n| Cell Metabolism | `\"Cell Metab\"[Journal]` |\n| Molecular Cell | `\"Mol Cell\"[Journal]` |\n\n### 已验证无用的参数\n- `sort=date` — 返回 warning \"Unknown sort schema 'date' ignored\"，无效果\n\n## 获取详情API (esummary)\n\n```python\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&id=<PMID列表>&retmode=json'\n```\n\n关键字段：\n- `pubtype` — 文章类型（含 \"Review\" 则为综述）\n- `source` — 期刊缩写名\n- `articleids[].idtype == 'doi'` — DOI\n- `attributes` — 包含 \"Has Abstract\" 则有摘要\n- `pubdate` — 发表日期\n- `title` — 标题\n\n## 获取摘要API (efetch)\n\n```python\n# XML模式（含结构化信息）\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=<PMIDs>&retmode=xml'\n\n# 文本模式（纯文本）\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=<PMIDs>&retmode=text&rettype=abstract'\n```\n\nXML模式返回结构化数据，适合用正则提取：\n- `<ArticleTitle>` → 标题\n- `<AbstractText>` → 摘要段落（可以有多段）\n- `<ELocationID EIdType=\"doi\">` → DOI\n- `<Journal><Title>` → 期刊全名（最后一个匹配是英文名）\n- `<PubDate><Year>` + `<Month>` → 发表日期\n\n## 获取文章元数据（XML完整模式）\n\n```python\nurl = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=<PMIDs>&retmode=xml'\n```\n此模式返回完整XML，包含：\n- 所有作者的 `<LastName>` 和 `<ForeName>`\n- 所有 `<Keyword>`\n- 完整 `<AbstractText>`\n- `<PublicationTypeList>` 用于排除Review/Letter\n\n## 已知环境陷阱\n\n### 1. SSL连接失败（exit code 35）\n某些环境（git-bash/MSYS2）的 curl SSL 版本与 NCBI 服务器不兼容。\n- 修复1：`curl -k`（跳过证书验证）\n- 修复2：改用 Python 的 `urllib.request`（在本环境中工作可靠）\n- 修复3：更新 curl CA bundle\n\n### 2. URL编码问题\ncurl 命令行中包含 `()\"[]` 等特殊字符时容易解析失败（exit code 3）。\n- 修复：用 Python 的 `urllib.parse.urlencode(params)` 构建查询串\n\n### 3. `sort=date` 无效\nPubMed API 不支持 sort=date，搜索结果的顺序按默认相关性排序。\n\n### 4. Cell/Elsevier 付费墙\nCell Press（www.cell.com、linkinghub.elsevier.com）对脚本访问严格限制，返回403/超时。Nature系列（www.nature.com）通常可正常访问。\n\n## 搜索策略（两阶段工作流）\n\n### 阶段A：主题搜索\n```python\nquery = 'Parkinson disease AND (dopamine neuron OR synuclein OR MPTP)'\nparams = {\n 'db': 'pubmed', 'term': query,\n 'mindate': '<3月前>', 'maxdate': '<今天>',\n 'datetype': 'pdat', 'retmax': 10, 'retmode': 'json'\n}\n```\n目的：了解该时段总论文数，获取基础PMID列表。\n\n### 阶段B：高影响力期刊过滤\n```python\njournal_tags = '\"Nature\"[Journal] OR \"Cell\"[Journal] OR \"Nat Neurosci\"[Journal] OR ...'\nquery = f'({topic_keywords}) AND ({journal_tags})'\n```\n目的：直接命中IF>10的高质量论文。\n\n### 初筛要点\n1. `pubtype` 不含 \"Review\" → 原创研究\n2. `attributes` 含 \"Has Abstract\" → 有摘要\n3. 检查 DOI 是否存在\n4. 排除 \"No authors listed\" → 通常是 News & Views\n\n## 评分标准（Claude评估用，5维度各1-10分）\n\n| 维度 | 评分要点 | 满分 |\n|------|---------|------|\n| 逻辑完整性 | gain+loss of function, KO/KI, 正反验证 | 10 |\n| 方法学水平 | 单细胞测序, 冷冻电镜, 活细胞成像, TurboID等 | 10 |\n| 创新性 | 新概念/新机制/新方法 | 10 |\n| 可借鉴性 | 实验设计可迁移到神经科学 **←最重要维度** | 10 |\n| 临床转化潜力 | 体内验证, 治疗靶点, 生物标志物 | 10 |\n\n总分 >= 30 且各维度 >= 5 → 候选。再经Hermes确认相关性后，只选最优1篇。\n", "file_type": ".md"}

{"todos": [{"id": "1", "content": "PubMed搜索外泌体/细胞外囊泡近期高质量论文", "status": "in_progress"}, {"id": "2", "content": "获取论文详情+摘要+初筛", "status": "pending"}, {"id": "3", "content": "Claude Code深度评估候选论文", "status": "pending"}, {"id": "4", "content": "选最优1篇并确认", "status": "pending"}, {"id": "5", "content": "下载PDF+提取Figure图片", "status": "pending"}, {"id": "6", "content": "制作HTML深度解读", "status": "pending"}, {"id": "7", "content": "生成推送内容", "status": "pending"}], "summary": {"total": 7, "pending": 6, "in_progress": 1, "completed": 0, "cancelled": 0}}

{"status": "error", "output": "Today: 2026/06/11, 3 months ago: 2026/03/13\r\n\r\n=== Phase A: Topic search ===\r\nURL: https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?db=pubmed&term=%28exosome%5BTitle%2FAbstract%5D+OR+%22extracellular+vesicle%22%5BTitle%2FAbstract%5D+OR+%22extracellular+vesicles%22%5BTitle%2FAbstract%5D%29+AND+%28neural%5BTitle%2FAbstract%5D+OR+brain%5BTitle%2FAbstract%5D+OR+neurodegeneration%5BTitle%2FAbstract%5D+OR+neuron%5BTitle%2FAbstract%5D+OR+microglia%5BTitle%2FAbstract%5D+OR+%22nervous+system%22%5BTitle%2FAbstract%5D%29&mindate=2026%2F03%2F13&maxdate=2026%2F06%2F11&datetype=pdat&retmax=15&retmode=json\r\nTotal results: 416\r\nPMIDs: ['42266184', '42265252', '42263287', '42262448', '42262353', '42261703', '42260749', '42257777', '42256442', '42256440', '42255476', '42254758', '42254188', '42254023', '42253953']\r\n\r\n=== Phase B: High-IF journal filter ===\r\nURL: https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?db=pubmed&term=%28%28exosome%5BTitle%2FAbstract%5D+OR+%22extracellular+vesicle%22%5BTitle%2FAbstract%5D+OR+%22extracellular+vesicles%22%5BTitle%2FAbstract%5D%29+AND+%28neural%5BTitle%2FAbstract%5D+OR+brain%5BTitle%2FAbstract%5D+OR+neurodegeneration%5BTitle%2FAbstract%5D+OR+neuron%5BTitle%2FAbstract%5D+OR+microglia%5BTitle%2FAbstract%5D+OR+%22nervous+system%22%5BTitle%2FAbstract%5D%29%29+AND+%28%22Nature%22%5BJournal%5D+OR+%22Cell%22%5BJournal%5D+OR+%22Nat+Neurosci%22%5BJournal%5D+OR+%22Neuron%22%5BJournal%5D+OR+%22Nat+Med%22%5BJournal%5D+OR+%22Nat+Commun%22%5BJournal%5D+OR+%22Sci+Adv%22%5BJournal%5D+OR+%22Cell+Rep%22%5BJournal%5D+OR+%22Cell+Death+Differ%22%5BJournal%5D+OR+%22EMBO+J%22%5BJournal%5D+OR+%22Mol+Neurodegener%22%5BJournal%5D+OR+%22Acta+Neuropathol%22%5BJournal%5D+OR+%22Brain%22%5BJournal%5D+OR+%22Lancet+Neurol%22%5BJournal%5D+OR+%22Dev+Cell%22%5BJournal%5D+OR+%22Cell+Metab%22%5BJournal%5D+OR+%22Mol+Cell%22%5BJournal%5D+OR+%22Nat+Struct+Mol+Biol%22%5BJournal%5D+OR+%22J+Clin+Invest%22%5BJournal%5D+OR+%22Nat+Rev+Neurosci%22%5BJournal%5D%29&mindate=2026%2F03%2F13&maxdate=2026%2F06%2F11&datetype=pdat&retmax=30&retmode=json\r\nTotal results: 10\r\nPMIDs: ['42192197', '42169139', '42153334', '41992946', '41991797', '41957395', '41927530', '41856111', '41132091', '41004427']\r\n\r\n=== Combined PMIDs: ['42266184', '42265252', '42263287', '42262448', '42262353', '42261703', '42260749', '42257777', '42256442', '42256440', '42255476', '42254758', '42254188', '42254023', '42253953', '42192197', '42169139', '42153334', '41992946', '41991797', '41957395', '41927530', '41856111', '41132091', '41004427'] ===\r\nTotal unique: 25\r\n\n--- stderr ---\nTraceback (most recent call last):\r\n File \"C:\\Users\\forgase\\AppData\\Local\\Temp\\hermes_sandbox_ajvkkaun\\script.py\", line 73, in <module>\r\n with open('/tmp/pubmed_search_results.json', 'w') as f:\r\n ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^\r\nFileNotFoundError: [Errno 2] No such file or directory: '/tmp/pubmed_search_results.json'\r\n", "tool_calls_made": 0, "duration_seconds": 1.42, "error": "Traceback (most recent call last):\r\n File \"C:\\Users\\forgase\\AppData\\Local\\Temp\\hermes_sandbox_ajvkkaun\\script.py\", line 73, in <module>\r\n with open('/tmp/pubmed_search_results.json', 'w') as f:\r\n ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^\r\nFileNotFoundError: [Errno 2] No such file or directory: '/tmp/pubmed_search_results.json'\r\n"}

{"status": "success", "output": "=== Batch 1 Papers ===\r\n\r\nPMID: 42266184\r\nTitle: Pathways of Protein Secretion in Prokaryotes and Eukaryotes: Molecular Mechanisms, Biological Functions, and Therapeutic\r\nJournal: MedComm (2020) | Date: 2026 Jun\r\nDOI: 10.1002/mco2.70798\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42265252\r\nTitle: Glia cell-derived extracellular vesicles as modulators in spinal cord injury repair.\r\nJournal: Spinal Cord | Date: 2026 Jun 9\r\nDOI: 10.1038/s41393-026-01231-z\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42263287\r\nTitle: Exosome-Rich Mesenchymal Stem Cell Secretome Improves Symptoms From Parkinson's Disease: A Case Series.\r\nJournal: Pain Med Case Rep | Date: 2026 May\r\nDOI: \r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42262448\r\nTitle: Bacterial Extracellular Vesicles as Systemic Messengers: Integrating Their Roles in Gut-Liver-Brain Communication and Di\r\nJournal: Probiotics Antimicrob Proteins | Date: 2026 Jun 9\r\nDOI: 10.1007/s12602-026-11083-x\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42262353\r\nTitle: Traumatic Brain Injury Enhances Susceptibility to Lung Bacterial Infection in Mice and Pigs by Modulating the Innate Imm\r\nJournal: Crit Care Med | Date: 2026 Jun 9\r\nDOI: 10.1097/CCM.0000000000007212\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42261703\r\nTitle: Blood-based biomarkers for dementia with Lewy bodies.\r\nJournal: J Alzheimers Dis | Date: 2026 Jun 9\r\nDOI: 10.1177/13872877261456317\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42260749\r\nTitle: Preeclampsia-derived small extracellular vesicles disrupt blood-brain barrier integrity and activate glial cells in vitr\r\nJournal: J Physiol | Date: 2026 Jun 8\r\nDOI: 10.1113/JP289761\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42257777\r\nTitle: Mapping the landscape and evolution of drug delivery for glioma: a bibliometric and visual analysis.\r\nJournal: Discov Oncol | Date: 2026 Jun 8\r\nDOI: 10.1007/s12672-026-05406-1\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42256442\r\nTitle: Extracellular Vesicle-Associated IL4 Displays Enhanced Anti-Inflammatory Properties in Microglial Cells.\r\nJournal: J Extracell Biol | Date: 2026 Jun\r\nDOI: 10.1002/jex2.70111\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42256440\r\nTitle: Extracellular Vesicles in the Biology and Liquid Biopsy Diagnostics of Pediatric High-Grade Glioma - Emerging Findings a\r\nJournal: J Extracell Biol | Date: 2026 Jun\r\nDOI: 10.1002/jex2.70152\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42255476\r\nTitle: Retraction: Extracellular vesicle-derived microRNA-410 from mesenchymal stem cells protects against neonatal hypoxia-isc\r\nJournal: Front Cell Dev Biol | Date: 2026\r\nDOI: 10.3389/fcell.2026.1868597\r\nType: ['Journal Article', 'Retraction Notice']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42254758\r\nTitle: Research on stem cell therapy for brain injury: A bibliometric and visual analysis (2009-2025).\r\nJournal: IBRO Neurosci Rep | Date: 2026 Jun\r\nDOI: 10.1016/j.ibneur.2026.04.015\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42254188\r\nTitle: Molecular regulators of thromboinflammation and angiogenesis in pediatric cancer: emerging roles of noncoding RNAs, epig\r\nJournal: Ann Med Surg (Lond) | Date: 2026 Jun\r\nDOI: 10.1097/MS9.0000000000004853\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42254023\r\nTitle: Ferroptosis: an emerging key mechanism linking aging, surgical and anesthetic exposure to postoperative cognitive dysfun\r\nJournal: Front Immunol | Date: 2026\r\nDOI: 10.3389/fimmu.2026.1836516\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42253953\r\nTitle: Glioma-derived extracellular vesicles as drivers of immunotherapeutic resistance: mechanisms of immune reprogramming and\r\nJournal: Front Immunol | Date: 2026\r\nDOI: 10.3389/fimmu.2026.1859282\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n", "tool_calls_made": 0, "duration_seconds": 0.83}

{"status": "success", "output": "=== Batch 2 (High-IF filter) Papers ===\r\n\r\nPMID: 42192197\r\nTitle: Microglial mitochondria transfer to astrocytes via GPNMB-enriched extracellular vesicles alleviates cognitive deficits in tauopathy mice.\r\nJournal: Nat Neurosci | Date: 2026 May 26\r\nDOI: 10.1038/s41593-026-02317-w\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42169139\r\nTitle: Mitochondria transfer in neurological disorders: the key role of neuroglia.\r\nJournal: Mol Neurodegener | Date: 2026 May 21\r\nDOI: 10.1186/s13024-026-00953-1\r\nType: ['Journal Article', 'Review']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 42153334\r\nTitle: Parkinson's disease beyond the brain: erythrocyte α-synuclein transfer across the blood-brain barrier.\r\nJournal: Brain | Date: 2026 May 19\r\nDOI: 10.1093/brain/awag179\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41992946\r\nTitle: Blood mtDNA markers of mitochondrial subtype and early-onset Parkinson's disease biology.\r\nJournal: Brain | Date: 2026 Apr 16\r\nDOI: 10.1093/brain/awag135\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41991797\r\nTitle: Consensus statement on microglial and macrophage functions in gliomas.\r\nJournal: Acta Neuropathol | Date: 2026 Apr 16\r\nDOI: 10.1007/s00401-026-02999-3\r\nType: ['Journal Article', 'Review', \"Research Support, Non-U.S. Gov't\"]\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41957395\r\nTitle: Steric hindrance-mediated extracellular vesicle size fractionation for rapid prehospital diagnosis of intracerebral hemorrhage.\r\nJournal: Nat Commun | Date: 2026 Apr 9\r\nDOI: 10.1038/s41467-026-71751-y\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41927530\r\nTitle: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.\r\nJournal: Nat Commun | Date: 2026 Apr 3\r\nDOI: 10.1038/s41467-026-70469-1\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41856111\r\nTitle: Transplantation of encapsulated mitochondria alleviates dysfunction in mitochondrial and Parkinson's disease models.\r\nJournal: Cell | Date: 2026 May 14\r\nDOI: 10.1016/j.cell.2026.02.023\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41132091\r\nTitle: EXOSC10 haploinsufficiency causes primary microcephaly by derepression of Sonic hedgehog signalling.\r\nJournal: Brain | Date: 2026 Jun 3\r\nDOI: 10.1093/brain/awaf405\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n\r\nPMID: 41004427\r\nTitle: MYC-driven gliosis impairs neuron-glia communication in amyotrophic lateral sclerosis.\r\nJournal: Brain | Date: 2026 May 5\r\nDOI: 10.1093/brain/awaf360\r\nType: ['Journal Article']\r\nHasAbstract: True\r\n---\r\n", "tool_calls_made": 0, "duration_seconds": 1.01}

{"status": "success", "output": "\r\n================================================================================\r\nPMID: 42192197\r\nJournal: Nature neuroscience\r\nTitle: Microglial mitochondria transfer to astrocytes via GPNMB-enriched extracellular vesicles alleviates cognitive deficits in tauopathy mice.\r\nDOI: 10.1038/s41593-026-02317-w\r\nTypes: ['Journal Article']\r\nKeywords: []\r\nAbstract: Alzheimer's disease (AD) is an irreversible neurodegenerative disease characterized by cognitive decline. The precise molecular mechanisms that underlie the pathogenesis of AD remain elusive. Here we show that glycoprotein nonmetastatic melanoma protein B (GPNMB) is produced by microglia and transferred to astrocytes through extracellular vesicles (EVs) in PS19 tau pathology mice. Tau is cleaved in microglia to generate N-terminal fragments that form a complex on mitochondria with Parkin/Nix and...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 41957395\r\nJournal: Nature communications\r\nTitle: Steric hindrance-mediated extracellular vesicle size fractionation for rapid prehospital diagnosis of intracerebral hemorrhage.\r\nDOI: 10.1038/s41467-026-71751-y\r\nTypes: ['Journal Article']\r\nKeywords: []\r\nAbstract: Diagnosing intracerebral hemorrhage (ICH) in prehospital settings remains challenging due to unavailability of immediate neuroimaging, clinical overlap with ischemic stroke, and absence of validated circulating biomarkers for time-critical settings. Extracellular vesicles (EVs), subcellular structures capable of transporting biomolecular payloads (e.g., proteins, nucleic acids) across the blood-brain barrier, have emerged as compelling diagnostic candidates for ICH. Nevertheless, their clinical ...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 41927530\r\nJournal: Nature communications\r\nTitle: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.\r\nDOI: 10.1038/s41467-026-70469-1\r\nTypes: ['Journal Article']\r\nKeywords: []\r\nAbstract: Extracellular vesicles (EVs) are cell-derived small membrane vesicles and circulate throughout the body, but the impact of circulating EVs on brain function and behavior remains elusive. Here, we report that wild-type (WT) mouse blood, particularly EVs, increases sociability in socially impaired immunodeficient Rag1-/- male mice, mimicking the effects of WT T cell transfer. These EVs are localized to neurons and regulate PKCε expression, GABAA receptor synaptic localization, and inhibitory posts...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 42260749\r\nJournal: The Journal of physiology\r\nTitle: Preeclampsia-derived small extracellular vesicles disrupt blood-brain barrier integrity and activate glial cells in vitro.\r\nDOI: 10.1113/JP289761\r\nTypes: ['Journal Article']\r\nKeywords: ['astrocytes', 'blood–brain barrier', 'microglia', 'organ‐on‐a‐chip', 'placenta–brain axis', 'preeclampsia', 'small extracellular vesicles']\r\nAbstract: Structural and functional integrity of the blood-brain barrier (BBB) is crucial for maintaining brain homeostasis. Preeclampsia, characterized by new-onset hypertension and endothelial dysfunction in pregnancy, often involves BBB disruption and neurological complications. Small extracellular vesicles (sEVs) have emerged as potential drivers of BBB disruption during preeclampsia; however, their impact on maternal brain health, particularly at the cellular level, remains understudied. Here, the ef...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 42256442\r\nJournal: Journal of extracellular biology\r\nTitle: Extracellular Vesicle-Associated IL4 Displays Enhanced Anti-Inflammatory Properties in Microglial Cells.\r\nDOI: 10.1002/jex2.70111\r\nTypes: ['Journal Article']\r\nKeywords: ['BV2', 'IL4 receptor', 'anti‐inflammatory effect', 'endosomal pathway', 'extracellular vesicles', 'interleukin‐4', 'kinetic']\r\nAbstract: Neuroinflammation and neurodegeneration are strictly related phenomena, characterized by dysregulation of microglia, central nervous system (CNS) resident immune cells. Interleukin-4 (IL4) has shown beneficial abilities to re-establish microglial homeostasis in experimental models of CNS traumatic injury, stroke and multiple sclerosis, but its optimal administration system remains uncertain. Here, we show that extracellular vesicles (EVs) released by engineered murine microglia BV2 cells constit...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 42153334\r\nJournal: Brain : a journal of neurology\r\nTitle: Parkinson's disease beyond the brain: erythrocyte α-synuclein transfer across the blood-brain barrier.\r\nDOI: 10.1093/brain/awag179\r\nTypes: ['Journal Article']\r\nKeywords: ['Parkinson’s disease', 'blood–brain barrier', 'bone marrow', 'red blood cells', 'synucleinopathies', 'α-synuclein']\r\nAbstract: Parkinson's disease is characterized by the accumulation and propagation of α-synuclein pathology in the central nervous system, yet the contribution of peripheral α-synuclein sources remains unclear. Here, we identify erythrocytes as an important reservoir of α-synuclein and demonstrate that bone marrow-derived erythrocytic α-synuclein likely contributes to brain pathology and Parkinson's disease-related neurodegeneration. Using human tissues and mouse models, we show that erythrocytes harbour ...\r\n================================================================================\r\n\r\n================================================================================\r\nPMID: 41856111\r\nJournal: Cell\r\nTitle: Transplantation of encapsulated mitochondria alleviates dysfunction in mitochondrial and Parkinson's disease models.\r\nDOI: 10.1016/j.cell.2026.02.023\r\nTypes: ['Journal Article']\r\nKeywords: ['Parkinson’s disease', 'degenerative disease', 'extracellular vesicle', 'mitochondria', 'mitochondrial diseases', 'mitochondrial transfer', 'mtDNA depletion syndrome', 'mtDNA mutation', 'organelle therapy']\r\nAbstract: Mitochondrial transplantation holds significant potential for the treatment of mitochondrial diseases. However, how to efficiently deliver exogenous mitochondria to somatic cells or tissues remains unresolved. We present a mitochondrial transplantation approach to deliver mitochondria into the cells and tissues of mice and monkeys with high efficiency, based on encapsulating mitochondria with vesicles derived from the plasma membrane of erythrocytes. Treatment with encapsulated mitochondria comp...\r\n================================================================================\r\n", "tool_calls_made": 0, "duration_seconds": 1.44}

{"status": "success", "output": "Title: Microglial mitochondria transfer to astrocytes via GPNMB-enriched extracellular vesicles alleviates cognitive deficits in tauopathy mice.\r\nAuthors: Chensi Liang, Yulan Zhou, Kai Zhuang, Shuzhong Wang, Li Zhong, Dan Can, Aiyu Lei, Huifang Li, Jie Zhang, Lige Leng\r\nFull Abstract:\r\n\r\nAlzheimer's disease (AD) is an irreversible neurodegenerative disease characterized by cognitive decline. The precise molecular mechanisms that underlie the pathogenesis of AD remain elusive. Here we show that glycoprotein nonmetastatic melanoma protein B (GPNMB) is produced by microglia and transferred to astrocytes through extracellular vesicles (EVs) in PS19 tau pathology mice. Tau is cleaved in microglia to generate N-terminal fragments that form a complex on mitochondria with Parkin/Nix and GPNMB, promoting the secretion of EVs containing mitochondria. Functional mitochondria transferred to astrocytes via EVs markedly improve astrocytic functions and attenuate the cognitive impairments and pathogenic features in PS19 mice. By contrast, microglial GPNMB deficiency eliminates mitochondrial EV secretion and mitochondrial transfer to astrocytes, thereby impairing astrocytic functions and exacerbating cognitive impairment in PS19-CcKO (CX3CR1 cre Gpnmb floxp) mice. GPNMB-enriched EVs from PS19 mice alleviate the pathological phenotypes of PS19 mice, offering potential insights for AD treatment.\r\n\r\nDOI: 10.1038/s41593-026-02317-w\r\n\r\nDOI: 10.1016/S1474-4422(19)30435-1\r\n\r\nDOI: 10.1515/revneuro-2022-0087\r\n\r\nDOI: 10.1186/s13024-017-0184-x\r\n\r\nDOI: 10.1097/NEN.0b013e3182933788\r\n\r\nDOI: 10.1083/jcb.201709069\r\n\r\nDOI: 10.1186/s13024-021-00473-0\r\n\r\nDOI: 10.1186/s13024-021-00440-9\r\n\r\nDOI: 10.3389/fphys.2012.00229\r\n\r\nDOI: 10.1038/nn.4132\r\n\r\nDOI: 10.1093/hmg/ddp367\r\n\r\nDOI: 10.1093/hmg/ddv059\r\n\r\nDOI: 10.1016/j.nbd.2013.10.018\r\n\r\nDOI: 10.3389/fimmu.2022.978571\r\n\r\nDOI: 10.1126/sciadv.abe5085\r\n\r\nDOI: 10.1038/ncomms9472\r\n\r\nDOI: 10.1038/s41467-022-31213-7\r\n\r\nDOI: 10.1126/science.abq4822\r\n\r\nDOI: 10.1016/j.cell.2019.04.001\r\n\r\nDOI: 10.1007/s00401-021-02372-6\r\n\r\nDOI: 10.1186/s40478-018-0612-3\r\n\r\nDOI: 10.1016/j.yexcr.2008.02.006\r\n\r\nDOI: 10.4049/jimmunol.178.10.6557\r\n\r\nDOI: 10.1016/j.jid.2017.08.034\r\n\r\nDOI: 10.1073/pnas.2316175121\r\n\r\nDOI: 10.1128/AAC.17.3.355\r\n\r\nDOI: 10.1038/nature18928\r\n\r\nDOI: 10.1038/s41593-018-0332-9\r\n\r\nDOI: 10.15252/embj.201899360\r\n\r\nDOI: 10.1038/s41467-023-40680-5\r\n\r\nDOI: 10.1186/s12974-018-1100-1\r\n\r\nDOI: 10.1038/nprot.2014.006\r\n\r\nDOI: 10.1126/science.abf1230\r\n\r\nDOI: 10.1038/nature25442\r\n\r\nDOI: 10.1038/s41586-018-0698-6\r\n\r\nDOI: 10.1016/j.brainresbull.2011.10.004\r\n\r\nDOI: 10.1126/science.abk0637\r\n\r\nDOI: 10.1038/srep23241\r\n\r\nDOI: 10.1007/s12035-017-0707-z\r\n\r\nDOI: 10.1016/j.cell.2021.12.041\r\n\r\nDOI: 10.1016/j.bbadis.2009.09.002\r\n\r\nDOI: 10.3389/fnins.2018.00342\r\n\r\nDOI: 10.1515/hsz-2012-0119\r\n\r\nDOI: 10.1007/s10495-024-01977-y\r\n\r\nDOI: 10.1083/jcb.202004029\r\n\r\nDOI: 10.1016/j.tcb.2016.05.008\r\n\r\nDOI: 10.1080/15548627.2019.1596479\r\n\r\nDOI: 10.3390/ijms241813835\r\n\r\nDOI: 10.1038/s41467-021-21984-w\r\n\r\nDOI: 10.1182/blood-2014-05-573543\r\n\r\nDOI: 10.1126/science.1093133\r\n\r\nDOI: 10.1016/j.redox.2025.103516\r\n\r\nDOI: 10.1186/s12933-025-02884-5\r\n\r\nDOI: 10.1101/gr.233866.117\r\n\r\nDOI: 10.1016/j.neuron.2007.01.010\r\n\r\nDOI: 10.1186/1742-2094-4-26\r\n\r\nDOI: 10.1002/jev2.12398\r\n\r\nDOI: 10.1126/sciadv.abe1340\r\n\r\nDOI: 10.1002/advs.202409105\r\n\r\nDOI: 10.1038/s42255-022-00643-4\r\nJournal: Nature neuroscience\r\nISO: Nat Neurosci\r\nDate: 2026-May-26\r\n", "tool_calls_made": 0, "duration_seconds": 0.81}

{"todos": [{"id": "1", "content": "PubMed搜索外泌体/细胞外囊泡近期高质量论文", "status": "completed"}, {"id": "2", "content": "获取论文详情+摘要+初筛", "status": "completed"}, {"id": "3", "content": "Claude Code深度评估候选论文", "status": "completed"}, {"id": "4", "content": "选最优1篇并确认", "status": "completed"}, {"id": "5", "content": "下载PDF+提取Figure图片", "status": "in_progress"}, {"id": "6", "content": "制作HTML深度解读", "status": "pending"}, {"id": "7", "content": "生成推送内容", "status": "pending"}], "summary": {"total": 7, "pending": 2, "in_progress": 1, "completed": 4, "cancelled": 0}}

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{"output": "Found 6 figure elements\r\n--- Figure Context 1 ---\r\nFig1\" class=\"c-article-section__figure-caption\" data-test=\"figure-caption-text\">Fig. 1: GPNMB increased in microglia of 5xFAD mice but in astrocytes of PS19 mice and patients with AD.\r\n\r\n--- Figure Context 2 ---\r\nFig1_HTML.png\" alt=\"Fig. 1: GPNMB increased in microglia of 5xFAD mice but in astrocytes of PS19 mice and patients with AD.\" loading=\"lazy\" width=\"685\" height=\"684\">\r\n\r\n--- Figure Context 3 ---\r\nFig2\" class=\"c-article-section__figure-caption\" data-test=\"figure-caption-text\">Fig. 2: GPNMB is produced by microglia and transferred from microglia to astrocytes in response to tau stimulation.\r\n\r\n--- Figure Context 4 ---\r\nFig2_HTML.png\" alt=\"Fig. 2: GPNMB is produced by microglia and transferred from microglia to astrocytes in response to tau stimulation.\" loading=\"lazy\" width=\"685\" height=\"558\">\r\n\r\n--- Figure Context 5 ---\r\nFig3\" class=\"c-article-section__figure-caption\" data-test=\"figure-caption-text\">Fig. 3: Microglial GPNMB deficiency aggravated cognition impairments in PS19 mice.\r\n\r\n--- Figure Context 6 ---\r\nFig3_HTML.png\" alt=\"Fig. 3: Microglial GPNMB deficiency aggravated cognition impairments in PS19 mice.\" loading=\"lazy\" width=\"685\" height=\"413\">\r\n\r\n--- Figure Context 7 ---\r\nFig4\" class=\"c-article-section__figure-caption\" data-test=\"figure-caption-text\">Fig. 4: GPNMB mediates the transfer of mitochondria from microglia to astrocytes through EVs.\r\n\r\n--- Figure Context 8 ---\r\nFig4_HTML.png\" alt=\"Fig. 4: GPNMB mediates the transfer of mitochondria from microglia to astrocytes through EVs.\" loading=\"lazy\" width=\"685\" height=\"575\">\r\n\r\n--- Figure Context 9 ---\r\nFig5\" class=\"c-article-section__figure-caption\" data-test=\"figure-caption-text\">Fig. 5: EVs captured by astrocytes mediate astrocyte reactivity and mitochondrial function.\r\n\r\n--- Figure Context 10 ---\r\nFig5_HTML.png\" alt=\"Fig. 5: EVs captured by astrocytes mediate astrocyte reactivity and mitochondrial function.\" loading=\"lazy\" width=\"685\" height=\"612\">", "exit_code": 0, "error": null}

{"output": "Found 6 figcaption elements\r\n\r\n=== Full Fig 1 Caption ===\r\nFig. 1: GPNMB increased in microglia of 5xFAD mice but in astrocytes of PS19 mice and patients with AD.\r\n\r\n\r\n=== Full Fig 2 Caption ===\r\nFig. 2: GPNMB is produced by microglia and transferred from microglia to astrocytes in response to tau stimulation.\r\n\r\n\r\n=== Full Fig 3 Caption ===\r\nFig. 3: Microglial GPNMB deficiency aggravated cognition impairments in PS19 mice.\r\n\r\n\r\n=== Full Fig 4 Caption ===\r\nFig. 4: GPNMB mediates the transfer of mitochondria from microglia to astrocytes through EVs.\r\n\r\n\r\n=== Full Fig 5 Caption ===\r\nFig. 5: EVs captured by astrocytes mediate astrocyte reactivity and mitochondrial function.\r\n\r\n\r\n=== Full Fig 6 Caption ===\r\nFig. 6: Exogenous supplementation of GPNMB-enriched EVs ameliorates the pathologic phenotypes of PS19 and PS19-CcKO mice.\r\n\r\nAria labels: 0", "exit_code": 0, "error": null}

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{"output": "Traceback (most recent call last):\r\n File \"C:\\Users\\forgase\\build_exosome_html.py\", line 14, in <module>\r\n with open(path, \"r\") as f:\r\n ^^^^^^^^^^^^^^^\r\nFileNotFoundError: [Errno 2] No such file or directory: '/c/Users/forgase/fig1_b64.txt'", "exit_code": 1, "error": null}

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{"success": true, "diff": "--- a/C:\\Users\\forgase\\build_exosome_html.py\n+++ b/C:\\Users\\forgase\\build_exosome_html.py\n@@ -10,7 +10,7 @@\n # ─── Read base64 figure data ───\n fig_b64 = {}\n for i in range(1, 7):\n- path = f\"/c/Users/forgase/fig{i}_b64.txt\"\n+ path = f\"C:/Users/forgase/fig{i}_b64.txt\"\n with open(path, \"r\") as f:\n fig_b64[f\"fig{i}\"] = f.read().strip()\n \n", "files_modified": ["C:\\Users\\forgase\\build_exosome_html.py"], "lint": {"status": "ok", "output": ""}, "resolved_path": "C:\\Users\\forgase\\build_exosome_html.py"}

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